Full Forms or Abbreviations Used In rDNA Technology

Recombinant DNA (rDNA) technology is a revolutionary field of biotechnology that has transformed how we study and manipulate genetic material. As with any specialized discipline, rDNA technology comes with its technical jargon and abbreviations essential for scientists and researchers to communicate effectively and understand the intricacies of their work. These abbreviations serve as shorthand for complex processes. Understanding these abbreviations is crucial for unlocking the potential of rDNA technology.

• rDNA - Recombinant DNA
• DNA - Deoxyribonucleic Acid
• RNA - Ribonucleic Acid
• PCR - Polymerase Chain Reaction
• RT-PCR - Reverse Transcription Polymerase Chain Reaction
• cDNA - Complementary DNA
• mRNA - Messenger RNA
• NGS - Next-Generation Sequencing
• SNP - Single Nucleotide Polymorphism
• CRISPR - Clustered Regularly Interspaced Short Palindromic Repeats
• TALEN - Transcription Activator-Like Effector Nucleases
• ZFN - Zinc Finger Nucleases
• LTR - Long Terminal Repeat
• LCR - Locus Control Region
• RFLP - Restriction Fragment Length Polymorphism
• ORF - Open Reading Frame
• GFP - Green Fluorescent Protein
• CAT - Chloramphenicol Acetyltransferase
• LacZ - Beta-Galactosidase
• Bla - Beta-Lactamase
• FISH - Fluorescence In Situ Hybridization
• EMSA - Electrophoretic Mobility Shift Assay
• ChIP - Chromatin Immunoprecipitation
• IP - Immunoprecipitation
• GST - Glutathione S-Transferase
• TGF-β - Transforming Growth Factor Beta
• BMP - Bone Morphogenetic Protein
• IRES - Internal Ribosome Entry Site
• siRNA - Small Interfering RNA
• shRNA - Short Hairpin RNA
• ASO - Antisense Oligonucleotide
• ASO-RNA - Antisense Oligonucleotide RNA
• TSD - Target Site Duplication
• LTR-R - Long Terminal Repeat Retrotransposon
• HAT - Hypoxanthine-Aminopterin-Thymidine
• EBV - Epstein-Barr Virus
• PAM - Protospacer Adjacent Motif
• RISC - RNA-Induced Silencing Complex
• DSB - Double-Strand Break
• ssDNA - Single-Stranded DNA
• dsDNA - Double-Stranded DNA
• DNA-PK - DNA-Dependent Protein Kinase
• T-DNA - Transfer DNA
• TSS - Transcription Start Site
• CDS - Coding Sequence
• UTR - Untranslated Region
• ITR - Inverted Terminal Repeat
• NHEJ - Non-Homologous End Joining
• HDR - Homology-Directed Repair
• PCR2 - Polymerase Cycling Assembly
• PEG - Polyethylene Glycol
• DMSO - Dimethyl Sulfoxide
• IPTG - Isopropyl β-D-1-thiogalactopyranoside
• LAMP - Loop-Mediated Isothermal Amplification
• HPLC - High-Performance Liquid Chromatography

• SDS-PAGE - Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis
• PAGE - Polyacrylamide Gel Electrophoresis
• RIA - Radioimmunoassay
• ELISA - Enzyme-Linked Immunosorbent Assay
• FACS - Fluorescence-Activated Cell Sorting
• NMR - Nuclear Magnetic Resonance
• MALDI-TOF - Matrix-Assisted Laser Desorption/Ionization Time-of-Flight
• EMS - Ethyl Methanesulfonate
• UV - Ultraviolet
• TMB - 3,3',5,5'-Tetramethylbenzidine
• BSA - Bovine Serum Albumin
• IPT - Isopropylthio-β-D-galactoside
• FRET - Fluorescence Resonance Energy Transfer
• BAC - Bacterial Artificial Chromosome
• YAC - Yeast Artificial Chromosome
• LCR - Loop-Mediated Isothermal Amplification
• qPCR - Quantitative Polymerase Chain Reaction
• dNTP - Deoxynucleotide Triphosphate
• NHEJ - Non-Homologous End Joining
• LAMP - Loop-Mediated Isothermal Amplification
• FISH - Fluorescent In Situ Hybridization
• FP - Green Fluorescent Protein
• CAT - Chloramphenicol Acetyltransferase
• HIS - Histidine
• AMP - Ampicillin
• CMV - Cytomegalovirus
• HIV - Human Immunodeficiency Virus
• HSV - Herpes Simplex Virus
• HBV - Hepatitis B Virus
• HCV - Hepatitis C Virus
• IAV - Influenza A Virus
• VSV - Vesicular Stomatitis Virus
• HIV - Human Immunodeficiency Virus
• PEG - Polyethylene Glycol
• CRISPRi - CRISPR Interference
• ZMW - Zero Mode Waveguide
• qRT-PCR - Quantitative Reverse Transcription Polymerase Chain Reaction
• RACE - Rapid Amplification of cDNA Ends
• TMB - Tetramethylbenzidine
• HRP - Horseradish Peroxidase
• EMS - Ethyl Methanesulfonate
• BAC - Bacterial Artificial Chromosome
• YAC - Yeast Artificial Chromosome
• ZFN - Zinc Finger Nucleases
• TALEN - Transcription Activator-Like Effector Nucleases

In conclusion, the world of recombinant DNA (rDNA) technology is both complex and fascinating, and using abbreviations plays a pivotal role in simplifying and streamlining communication within this field. The abbreviations discussed in this introduction are just a glimpse into the extensive lexicon of rDNA technology. Still, they represent the essential building blocks of genetic engineering, molecular biology, and biotechnology.

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